The immune system is composed of a variety of different cell types and proteins. Each element performs a specific task aimed at recognizing and/ or reacting against foreign material such as bacteria, viruses, parasites, and other pathogens. The body’s defenses consist of numerous different types of white blood cells that include lymphocytes, monocytes, and granulocytes. Lymphocytes are in turn further subdivided into T cells, B cells, and NK cells. The bone marrow and thymus represent training grounds for two cells of the adaptive immune system (B cells and T cells, respectively) which circulate the bloodstream and move around in our tissues, patrolling for signs of infection or tissue damage.
Identification of each cell type is essential for understanding their specific roles and conducting research in the field of immunology. T and B lymphocytes are two major adaptive immune cells in our body’s defense systems. However, the similar size and shape of the cells make it challenging to distinguish them. Currently, distinguishing different cell types is done by staining cells using fluorescent antibodies that bind to different clusters of differentiation (CD) receptors on the cell surface.
Now, a team led by Professor Chang Young-Tae at the Center for Self-assembly and Complexity within the Institute for Basic Science in Pohang, South Korea, successfully developed a small molecule probe CDyB (which stands for Compound of Designation yellow for B cell) that can accomplish live B cell distinction from T cells. CDyB was discovered using an unbiased fluorescence library screening called Diversity Oriented Fluorescence Library, or DOFL. By using this process, the researchers were able to screen for thousands of different molecules for their specificity to one type of immune cell over another. When applied to a mixture of T and B cells, this new probe was found to have high selectivity toward B cells.
CDyB is a new type of probe that does not require CD-specific antibodies to distinguish different cell types. Rather, it was found to be capable of entering the cell itself and staining the endoplasmic reticulum (ER) and Golgi apparatus, which are prominent organelles within the cells that are responsible for transporting materials within the cells. This is thought to be possible thanks to the molecule’s ability to easily pass through cell membranes.
After realizing that CDyB is localized within the ER / Golgi organelles, the researchers speculated that the mechanism of the B cell selectivity is based on gating. In other words, some transporter molecules must be responsible for the uptake and accumulation of the CDyB inside the organelles in some cells, but not the others. Hence they coined the new term, gating-oriented live-cell distinction (GOLD) to describe this newly discovered mechanism of distinguishing different types of cells.
Next, the researchers sought to find out why CDyB only stains the organelles of the B cells, but not T cells. The researchers further explored the mechanism of the new probe by using an SLC-CRISPR-based library, which is a platform that offers a high chance of systematic gating target elucidation. By employing SLC-CRISPRa and SLC-CRISPRi, the researchers discovered that SLC35C2 was the transporter specific for CDyB, which allows the molecule to be transported inside the organelles. The target transporter was further validated by the gene expression analysis. The researchers performed further knockout experiments and showed that deleting the transporter removed the ability of the molecule to be internalized by the ER / Golgi of the target cells, proving the role of SlC35C2 for the B cell selectivity.
Interestingly, the researchers observed that CDyB signal was stronger in mature B cells than in immature B cells. This is most likely due to the expression of SLC35C increasing according to the maturity of the B cells. The progenitor cells such as hematopoietic stem cells (HSC) and common lymphoid progenitor (CLP) express a low level of SLC35C2 and are thus minimally stained by CDyB. When they differentiate into T and NK cells, the expression of SLC35C2 remains low, hence yielding weak CDyB fluorescence. If the cells differentiate into B cell lineages, the SLC35C2 expression increases during the maturation path. The partially differentiated B-cell progenitors (Pre-Pro B, Pro B, Pre B) exhibit moderate CDyB fluorescence, and the fully matured B cells display the highest level of CDyB fluorescence.
Notably, Professor Chang’s team previously unlocked another B-cell-selective probe called CDgB (Compound of Designation green for B Cells) last year. Unlike CDyB, it distinguishes B cells over T cells using the Lipid-Oriented Live-cell Distinction (LOLD) mechanism. LOLD utilizes the small difference in membrane components, such as carbon chain length and cholesterol contents, and flexibility for cell discrimination. While CDyB showed stronger fluorescence in mature B cells, CDgB showed the brightest staining in immature B cells due to their softer membrane structure. It is hoped that utilizing both of these molecules with mechanisms together can be an effective way to distinguish different cell types in blood cells.
This study enriches the molecular probe toolbox and molecular understanding for live B cell distinction and opens the possibility of multi-dimensional cell analysis based on the orthogonal mechanism with new insight.
Dr. Min Gao,Sun Hyeok Lee,Dr. Raj Kumar Das,Dr. Haw-Young Kwon,Dr. Heon Seok Kim,Prof. Young-Tae Chang . A SLC35C2 Transporter‐Targeting Fluorescent Probe for the Selective Detection of B Lymphocytes Identified by SLC‐CRISPRi and Unbiased Fluorescence Library Screening, Angewandte Chemie International Edition (2022). DOI: 10.1002/anie.202202095