A new strategy to develop the disposable label-free immunosensor with electrochemiluminescent probing

Journal Reference

Journal of Electroanalytical Chemistry, Volume 747, 2015, Pages 136–142.

Lin Jiang¹, Ya Yang^1,2 , Yifeng Tu^1,3

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Institute of Analytical Chemistry, Soochow University, Dushu Lake Campus of Soochow University, Suzhou Industrial Park, 215123 Suzhou, PR China
Department of Forensic Medicine, Soochow University, Dushu Lake Campus of Soochow University, Suzhou Industrial Park, 215123 Suzhou, PR China
The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, Dushu Lake Campus of Soochow University, Suzhou Industrial Park, 215123 Suzhou, PR China

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Abstract

In this paper, a new strategy to prepare the label-free immunosensor signaling with the electrochemiluminescence (ECL) of luminol will be reported. The gold nano-particles (AuNPs) functionalized indium tin oxide coated glass is used as the substrate for antibody immobilization. These AuNPs also improve the ECL behavior of luminol. This strategy for immunosensor construction is simple, fast, cheap, and with no requirements of expensive apparatus. For validating the practicability of this new strategy, with methamphetamine (MA) as target model, an methamphetamine sensor was built by immobilizing the methamphetamine-antibody onto the surface of previously mentioned substrate. The ECL intensity on resultant sensor, after the direct immuno-interaction, decreased proportional to the concentration of methamphetamine in a wide linear range from 2 to 500 ng mL⁻¹ with a detection limit of 0.3 ng mL⁻¹. This proposed sensor is sensitive, specific, stable and reliable. It has been successfully applied to detect the methamphetamine in spiked human serums.

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