Nakayama N1, Hagiwara K1,2, Ito Y1, Ijiro K1,3, Osada Y1, Sano K1.[expand title=”Show Affiliations”]
- Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama 351-0198, Japan.
- Tokyo Metropolitan Institute of Medical Science, Setagaya, Tokyo 156-8506, Japan.
- Research Institute for Electronic Science, Hokkaido University, Sapporo, Hokkaido 001-0021, Japan [/expand].
Numerous cationic peptides that penetrate cells have been studied intensively as drug delivery system carriers for cellular delivery. However, cationic molecules tend to be cytotoxic and cause inflammation, and their stability in the blood is usually low. We have previously demonstrated that a rigid and fibrous cationic coiled-coil protein exhibited cell-penetrating ability superior to that of previously reported cell-penetrating peptides. Making use of structural properties, here we describe the cell-penetrating activity of a rigid and fibrous coiled-coil protein with a noncationic surface. A fibrous coiled-coil protein of pI 6.5 penetrated 100% of the cells tested in vitro at a concentration of 500 nM, which is comparable to that of previously reported cell-penetrating peptides. We also investigated the effect of cell-strain dependency and short-term cytotoxicity.Go To Langmuir.
Figure Legend: Summary of cell-penetrating activity of artificial protein having rigid and anisotropic structure with various surface properties.