A study conducted by researchers from Huazhong University of Science and Technology, Wuhan, China developed a highly specific new method for detection of telomerase activity from cultured tumor cells and primary cell specimens. The study is as the subsequent work in this systematic study of telomerase activity detection. This work maintains simplicity, high sensitivity, and rapidity. Furthermore, by drastically decreasing the fluorescence background, it obtains more remarkable specificity in contrast to the previous work. Urine specimens from 38 bladder cancer patients and 15 normal people are successfully detected using this method. Thus, this modified strategy could provide an efficient new tool with higher specificity and reliability for detection of telomerase activity and clinical diagnosis of cancer.
Zhuang Y1, Zhang M1, Chen B2, Duan R1, Min X1, Zhang Z1, Zheng F3, Liang H3, Zhao Z2, Lou X1, Xia F1.
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- 1Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology , Wuhan 430074, China.
- 2State Key Laboratory of Luminescent Materials and Devices, South China University of Technology , Guangzhou 510640, China.
- 3Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan 430022, China.
Telomerase is a widely used tumor biomarker for early cancer diagnosis. On the basis of the combined use of aggregation-induced emission (AIE) fluorogens and quencher, a quencher group induced high specificity strategy for detection of telomerase activity from cell extracts and cancer patients’ urine specimens was creatively developed. In the absence of telomerase, fluorescence background is extremely low due to the short distance between quencher and AIE dye. In the addition of telomerase, fluorescence enhances significantly. The telomerase activity in the E-J, MCF-7, and HeLa extracts equivalent to 5-10 000 cells can be detected by this method in ∼1 h. Furthermore, the distinguishing of telomerase extracted from 38 cancer and 15 normal urine specimens confirms the reliability and practicality of this protocol. In contrast to our previous results (Anal. Chem. 2015, 87, 6822-6827), these advanced experiments obtain more remarkable specificity.Go To Anal Chem.