Development of localized surface plasmon resonance biosensors for the detection of Brettanomyces bruxellensis in wine

About the author

Marisa Manzano was born in Udine, Italy. She got the degree in Natural Sciences at University of Padua. She was researcher in Food Microbiology at the University of Udine at the Department of Food Science from 1990 till 2005. Since 2005 Associate Professor at University of Udine teaching: Molecular Biology Techniques, Biotechnology of Microorganisms, Genetic of Microorganisms, Selection and Use of Enological Yeasts in the degree course of Food Science and Technology and Viticulture and Enology. She is Member of Committees: Academic Collegiate of Doctorate Research, Didactic Committee, State Exam Commission; member of the Committee “Quality System in the laboratory of Microbiology” (UNICHIM). Co-author of a patent – detection of Listeria monocytogenes by PCR, 1996, C12Q. She has long experience in Microbiology and Molecular Biology. Now working on the development of DNA probes for the construction of biosensors for the detection of pathogens. She was invited speaker in various international conferences with talks about rapid detection of pathogens using molecular methods or biosensors. Author of 271 publications including papers/book chapter/abstracts and posters.

About the author

Priya Vizzini was born in India in 1985. She obtained bachelor’s degree in Biomedical Laboratory Technician at the University of Udine (Italy) in 2011. She won an Erasmus grant to spend 5 months at Troyes University of technology (France) working on biological applications of QCM and Localized Surface Plasmon Resonance biosensor under the supervision of the Prof. Rodica Elena Ionescu. She got the master’s degree in Plant and Animal Biotechnology in 2014 at the University of Udine (Italy). In december 2015 she was a selected speaker at the “2^thPARMA NANO-DAY”scientific workshop for the session agribusiness, ambient and biotechnology at Parma University. At present she collaborates in the Department of Agricultural, Food, Environmental and Animal science in University of Udine. She works on the development of molecular biology methods (PCR, Nested-PCR, DGGE/ TTGE and blotting techniques) for the detection of pathogens in food-beverage and on the evaluation of sterilization systems for private companies.

About the author

Kun Jia received his B.S. and M.S. degree in Chemistry from University of Electronic Science and Technology of China (UESTC) in 2007 and 2010, respectively. He obtained his Ph.D. degree from Laboratory of Nanotechnology and Instrumentation Optics (LNIO) at University of Technology of Troyes (UTT) in France under the supervision of Prof. Elena Rodica IONESCU at the end of 2013. In March 2014, Dr. JIA joined the School of Microelectronics and Solid State Electronics of UESTC as an associate professor in Chemistry. His current research interests lie in the field of plasmonic controlled polymer fluorescence, optical (bio)chemical sensors on flexible substrate, and synthesizing of advanced optofunctional polymeric nanocomposites.

About the author

Pierre-Michel Adam has obtained his PhD in Physics in 1995 (Université de Bourgogne), and is at present Full Professor at the Université de Technologie de Troyes (ICD-LNIO). Title of his PhD is “Photon Scanning Tunneling Microscope (PSTM) with a polychromatic incoherent light source. Near-field investigation of test samples and surface plasmons.” In january 1995, P.M. Adam has joined the Universite de Technologie de Troyes as an Assistant Professor and has been appointed Full professor in February 2003. His fields of research are near-field microscopy and spectroscopy, surface plasmons, surface enhanced Raman scattering. He is currently in charge of a research group “Nanospectroscopy” at the ICD-LNIO. He is vice-president of a European network COST action MP1302 “Nanospectroscopy”. He is author/coauthor of 76 publications in international journals. He is/has been the supervisor of 16 PhD thesis.

About the author

Rodica Elena Ionescu earned one Ph.D. degree from the Ben-Gurion University of Negev, Israel in Biotechnological Engineering in 2004 and a second Ph.D. degree in Chemistry, from the University of Bucharest, in 2007. She has performed three post-doctoral positions in France in the field of electrochemical biosensors. Between February-October 2008, Dr. Ionescu was a researcher fellow at the National Nanotechnology Laboratory, Lecce (Italy) working on impedimetric cell-chips based interdigitated microlectrodes. In November 1^st 2008, Dr. Ionescu joined the Université de Technologie de Troyes (UTT) as an Assistant Professor, becoming a member of the Laboratoire de Nanotechnologie et d’Instrumentation Optique (LNIO). In December 4^th 2009, Dr. Ionescu obtained the Habilitation Title for conducting independent research (HDR) equivalent to an Associate Professor position. Thanks to the obtained research grants, Dr. Ionescu developed novel acoustic and optical platforms for ultrasensitive detection of biomolecules and pesticides. In 2012, Dr. Ionescu was awarded with a national OSEO Innovation grant. Between April 1^st 2014 to March 30, 2015 Dr. Ionescu was the research manager of a Proof-of Concept (POC) Grant-NRF-POC 002-026 supported by the Singapore National Research Foundation concerning the Electrochemical lateral flow biosensor detection and quantification of Dengue virus in whole blood at the Nanyang Technological University, School of Materials Science and Engineering under an international CREATE-NTU-HUJ-BGU program. Her current research activities include the development of multi-analyte biosensing platforms, specific (bio)functionalization of surfaces, atomic force microscopy nanopipette applications, controllable synthesis of nanoparticle, evaluation of nanoparticles and water pollutants toxicity to living cells and microorganisms. She has published over 40 pre-reviewed articles, 4 book chapters and 7 patents with 9 extensions.

Journal Reference

Sensors and Actuators B: Chemical, Volume 223, February 2016, Pages 295–300 .

Marisa Manzano¹, Priya Vizzini¹,Kun Jia^2,3,Pierre-Michel Adam²,Rodica Elena Ionescu²

[expand title=”Show Affiliations”]

Department of Food Science, University of Udine, via Sondrio 2/A, 33100 Udine, Italy
Laboratoire de Nanotechnologie et d’Instrumentation Optique, Institute Charles Delaunay, Universite’ de Technologie de Troyes, UMR CNRS 6281, 12 Rue Marie-Curie, CS 42060, 10004 Troyes Cedex, France
School of Microelectronics and Solid-State Electronics, University of Electronic Science and Technology of China, 610054 Chengdu, China

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Abstract

Incident light interacting with noble-metal nanoparticles with smaller sizes than the wavelength of the incident light induces localized surface plasmon resonance (LSPR). In this work a gold nanostructured surface was used for the immobilization of a 5′ end Thiol modified DNA probe to develop a LSPR nanobiosensor for the detection of the spoiler wine yeast Brettanomyces bruxellensis. Gold was evaporated to obtain a gold thickness of 4 nm. DNA (2 μL) from the target microorganism and the negative control at various concentrations were used to test the specificity and sensitivity of the LSPR technique. Changes in the optical properties of the nanoparticles due to DNA-probe binding are reflected in the shift of LSPR extinction maximum (λ_max). The results obtained using as target microorganism B. bruxellensis, and as negative control Saccharomyces cerevisiae demonstrated the specificity of both the DNA-probe and the protocol. The LSPR spectrophotometry technique detects 0.1 ng/μL DNA target confirming the possibility to utilize this system for the detection of pathogen microorganisms present in low amount in food and beverage samples.

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