As an emerging material, nanogel system is hydrogel nanoparticle, its ideal fluid-like transport properties and hydrophilic environment make the nanogel a perfect candidate for bio-catalysis, biomedical diagnosis and therapy. The immobilization (encapsulation) of enzymes within the gel matrix provides protection from structural alterations and subsequent deactivation of the enzymes, ensuring higher loadings and better substrate mobility for efficient catalysis. In addition, the unique hydrophilic environment and water-rich structure of this nanogel make it an ideal probe for magnetic resonance (MR) imaging, where the signal detected is directly related to relaxation time.
In this work, focused on exploiting the structure-function relationships of hydrogel, the dual-enzyme loaded multifunctional magnetic nanogel, which is composed of functionalized superparamagnetic iron oxide (SPIO) particles, a dual enzyme species (catalase and superoxide dismutase) and a polysaccharide cationic polymer glycol chitosan gel, was developed to be an efficient and safe probe for dual-modality pathological responsive ultrasound (US) and T2-weighted MR imaging both in vitro and in vivo.
Wang X1, Niu D2, Li P3, Wu Q1, Bo X3, Liu B3, Bao S1, Su T1, Xu H3, Wang Q1.[expand title=”Show Affiliations”]
- Department of Chemistry and Advanced Research Institute, Tongji University, Shanghai, 200092, China.
- Lab of Low-Dimensional Materials Chemistry, Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, 200237, China.
- Department of MedicalUltrasound, Shanghai Tenth People’s Hospital, Tenth People’s Hospital of Tongji University, Shanghai, 200072, China.
A dual-enzyme-loaded multifunctional hybrid nanogel probe ([email protected]/acryl/biotin-CAT/SOD-gel, or SGC) has been developed for dual-modality pathological responsive ultrasound (US) imaging and enhanced T2-weighted magnetic resonance (MR) imaging. This probe is composed of functionalized superparamagnetic iron oxide particles, a dual enzyme species (catalase and superoxide dismutase), and a polysaccharide cationic polymer glycol chitosan gel. The dual-modality US/MR imaging capabilities of the hybrid nanogel for responsive US imaging and enhanced T2-weighted MR imaging have been evaluated both in vitro and in vivo. These results show that the hybrid nanogel SGC can exhibit efficient dual-enzyme biocatalysis with pathological species for responsive US imaging. SGC also demonstrates increased accumulation in acidic environments for enhanced T2-weighted MR imaging. Further research on these nanogel systems may lead to the development of more efficient US/MR contrast agents.Go To ACS Nano.
Figure legend: Dual-Enzyme Loaded Hybrid Nanogel System for Pathological Responsive US and MR imaging.